SH-AQ-Fumonisin
AgraQuant® Fumonisin ELISA test
Quantitative
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Description & Properties
AgraQuant® FUM test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented. These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples. With the AgraQuant® ELISA, up to 6 mycotoxins can be analyzed from 1 extraction, substantially shortening overall time-to-result.
| Quantitation range | 250-5000 ppb |
|---|---|
| Limit of detection | 200 ppb |
| Limit of quantitation | 250 ppb |
| Product/Environment | Product Testing |
| Storage Temperature | 2°C - 8°C |
| Result type | Quantitative |
| Storage Temperature | 2°C - 8°C |
| Technology/Application | ELISA |
| Industry/Market | Animal Feed, Aquaculture & Seafood, Food & Beverage, Laboratory, University & Research, Milling & Grain, Petfood |
| Commodity | Grains, nuts |
Materials
Materials supplied in the standard product
- antibody coated microwells in a microwell holder
- non-coated dilution microwells
- 5 vials of each fumonisin standard
- 1 bottle of fumonisin conjugate
- 1 bottle of substrate solution
- 1 bottle of stop solution
Materials required but not supplied
- Romer Series II® Mill or equivalent
- balance
- 400 g
- blender or a tightly sealing jar with lid
- graduated cylinder: 100mL
- 70% methanol (ACS grade)
- distilled or de-ionized water
- container for filtrate collection with minimum 125mL capacity
- Whatman#1 filter paper
- or equivalent
- filter funnel
- 8-channel and single channel pipettes capable of pipetting 50 µL
- 100µL
- 200µL and 1000µL with tips
- timer
- wash bottle
- absorbent paper towels
- 3 reagent boats for 8-channel pipettes
- microwell reader with a 450nm filter and an optional differential filter of 630nm or equivalent